快速成功建立兔腹主动脉狭窄的模型_腹主动脉狭窄
时间：2019-04-04 03:17:07 来源：雅意学习网 本文已影响 人 
【摘要】 目的 复制动脉粥样硬化狭窄的模型，血管造影测量动脉狭窄程度以证实造模成功。方法 实验于2003年2~5月在郑州大学人民医院心血管内科实验室完成。选择雄性4月龄新西兰大白兔20只，随机分为单纯饲喂高脂饮食组、高脂饮食同时动脉内膜剥脱组，每组10只。分笼饲养，饲喂正常的全价颗粒饲料，自由饮水，观察1周后进入实验。1周后所有的兔子均开始高脂饮食(含1.5%胆固醇)喂养，高脂饮食同时动脉内膜剥脱组高脂喂养3 d后用股动脉穿刺法行腹主动脉内皮剥脱术。术后继续喂养同样的高脂饲料。于术后第9周行腹主动脉血管造影检查，记录腹主动脉狭窄程度。结果 术后第9周进行血清总胆固醇、甘油三酯、高密度脂蛋白、低密度脂蛋白胆固醇测定，两组兔血清总胆固醇、甘油三酯、高、低密度脂蛋白胆固醇均升高，高脂饮食与动脉内膜剥脱组升高更明显(P
【关键词】 膳食;动脉粥样硬化;动物模型;血管造影术
Successfully and quikly establishing atherosclerosis models of stenosis in the obdominal aorta in rabbits YU Jie, GAO Chuanyu, XU Wenke, et al.Internal Medicine 1 Section, Department of Gerontology, First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052，China
【Abstract】 Objective To establish models of atherosclerosis and stenosis of rabbits, and evaluate the degree of stenosis by angiography.Methods The experiment was carried out in the laboratory of Department of Cardiovascular Medicine, Peoples Hospital of Zhengzhou University from February to May 2003.Twenty male New Zealand rabbits aged 4 months were fed with a standard diet in separate cages for 1 week without inhibiting drinking. Twenty New Zealand rabbits were randomly divided into highfat diet and highfat diet combined with arterial injury groups with 10 animals in each group.One week later, all the rabbits were fed with cholesterolsupplemented diet (1.5% cholesterol) and rabbits of highfat diet combined with arterial injury groups were subject to balloon catheter injury of the abdominal aorta by puncturing into femoral artery after 3 days of cholesterolfed. Rabbits were continuing fed with cholesterolrich diet after the operation. Angiography were used to record the degree of stenosis of the abdominal aorta at the 9th week after operation.Results All the 20 rabbits entered the result analysis. ①After 9 weeks,the levels of TC,TG,HDL,LDL were increased,which was more obviously in the highfat diet combined with arterial injury groups. ② Stenosis of abdominal aorta was observed at the 9th week by angiography and the rate of stenosis was 50%~70% ［(57.000±7.888)%］ and it was 8~23 mm in length. Conclusion The animal model of stenosis can be successfully established in a short time by balloon endothelial denudation of abdominal aorta combined with cholesterolfed. Angiography can accurately measure the degree of stenosis of the abdominal aorta.
【Key words】 Meal;Atherosclerosis;Animalmodel;Angiography
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作者单位：450052 郑州大学第一附属医院老年综合一科(于洁)；郑州大学人民医院心血管科(高传玉 许文克)，超声科(张连仲) 动脉粥样硬化是心血管系统中最常见的一种非炎症性、退行性和增生性病变，其特征性病理改变为动脉壁硬化及内膜粥样斑块形成，甚至狭窄形成。兔动脉解剖结构与人类相似，是理想的动物模型，可用于疾病的预防、治疗的研究。本文用21 g穿刺针穿刺兔股动脉，植入5F桡动脉鞘管，用人冠状动脉经皮冠状动脉介入球囊剥脱腹主动脉内膜同时饲喂高胆固醇饲料进行综合制模后，用血管造影测量动脉狭窄程度，以确认造模成功。
